Journal: American Journal of Clinical and Experimental Urology
Article Title: Th17 cells promote tumor growth in an immunocompetent orthotopic mouse model of prostate cancer
doi:
Figure Lengend Snippet: Immunohistochemical staining of IL-17 in the prostate tumors. A. IL-17 staining in MPC3-luc only, control, and test groups; magnification: 40 × and scale bar = 1000 µm; of the insets, 400 × and scale bar = 100 µm; arrows indicate the IL-17-positive lymphocytes. B. Quantification of the number of IL-17-positive lymphocytes per high power field (HPF) in the prostate tumors; error bars represent SEM; *P < 0.05 compared to either the control group or MPC3-luc only group.
Article Snippet: The following primary antibodies were used: goat anti-IL-17 polyclonal antibodies (E-19, sc-6077; 1:100 dilution), rabbit anti-NOS2 [inducible nitric oxide synthase (iNOS), N-20, sc-651; 1:100 dilution], and goat anti-arginase I (V-20, sc-18345; 1:100 dilution) were purchased from Santa Cruz Biotechnology, Inc., Dallas, TX, USA.
Techniques: Immunohistochemical staining, Staining